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ACCURATE, RAPID AND CONVENIENT SINGLE-STEP DISEASE DIAGNOSTIC METHOD USING SELF-AMPLIFICATION PRINCIPLE OF DETECTION SIGNAL
ACCURATE, RAPID AND CONVENIENT SINGLE-STEP DISEASE DIAGNOSTIC METHOD USING SELF-AMPLIFICATION PRINCIPLE OF DETECTION SIGNAL
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机译:使用检测信号自放大原理的准确,快速,便捷的单级病诊断方法
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摘要
A method for detecting a disease marker using self-amplification of a detection signal is disclosed. The method can include (a) a step of simultaneously inducing an antigen-antibody immune response and an Au particle formation reaction by reduction of Au ions in an assay solution prepared by, to a pre-assay solution in which all of an antibody or antigen for detection of a disease-specific marker, free Au ions, and adsorbed Au ions are present, adding a sample, which contains a disease-specific antigen or antibody binding specifically to the antibody or the antigen, and a reducing agent; and (b) a step of confirming the presence or absence of a disease-specific marker by a chromogenic reaction through the Au particle formation.
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