首页> 外国专利> METHOD FOR MICROBIOLOGICAL DETECTION BASED ON EXOSOME NUCLEIC ACID AND APPLICATION THEREOF

METHOD FOR MICROBIOLOGICAL DETECTION BASED ON EXOSOME NUCLEIC ACID AND APPLICATION THEREOF

机译:基于外显子核酸的微生物检测方法及其应用

摘要

The present invention provides a method for microbial detection based on exosome nucleic acid and application thereof. The method comprises: (a) separating and obtaining exosome nucleic acid from a sample to be tested, the sample to be tested coming from first species; (b) sequencing the exosome nucleic acid to obtain a sequencing resu (c) excluding nucleic acid sequences corresponding to the first species from the sequencing result, so as to obtain sequence data subjected to removal treatment; and (d) comparing the sequence data subjected to removal treatment with nucleic acid sequences in a microbial database to obtain a microbial detection result in the sample.
机译:本发明提供了基于外来体核酸的微生物检测方法及其应用。该方法包括:(a)从待测样品中分离并获得外来体核酸,所述待测样品来自第一物种; (b)对外泌体核酸进行测序以获得测序结果; (c)从测序结果中排除与第一物种相对应的核酸序列,以得到经过去除处理的序列数据; (d)将经过去除处理的序列数据与微生物数据库中的核酸序列进行比较,以获得样品中的微生物检测结果。

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