METHOD FOR PRODUCING NEURAL CREST CELLS AND SYMPATHETIC NEURONS

摘要

Pluripotent stem cells are cultured in a culture solution containing FGF2, retinoic acid and BMP4 to thereby produce neural crest cells. The neural crest cells are cultured in a culture solution containing a cytokine and BMP4 to thereby produce sympathetic neuron precursors. Further, the sympathetic neuron precursors are cultured in a culture solution containing a neurotrophic factor to thereby produce sympathetic neurons.