How to Enhance Plant Resistance to Invasive DNA Viruses

摘要

The present invention provides a method for enhancing plant tolerance to DNA viruses comprising the steps of: 1) contacting a target sequence conforming to the 5'-NX-NGG-3 'or 5'-CCN-NX-3' Selecting from the genomic sequence of the DNA virus and synthesizing a reverse-complementary DNA fragment in the target sequence; 2) constructing a DNA fragment in a vector to be used for expression of CRISPR / Cas9 nuclease, obtaining a recombinant vector capable of transcribing the guide RNA and realizing expression of the Cas9 protein; 3) introducing the recombinant vector into the awarded plant. The method of the present invention can be widely applied to single-stranded DNA viruses using double-stranded DNA viruses and double-stranded DNAs as intermediate products, in which the sequence of DNA viruses is known.