RNA- DNA RNA- METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION

摘要

This disclosure provides a DNA-targeted RNA comprising the target sequence and, in conjunction with the modified polypeptide, provides site specific modifications of the target DNA and / or polypeptide associated with the target DNA. The present disclosure further provides site-specific modified polypeptides. The present disclosure further provides a site-specific modification of the target DNA and / or the polypeptide associated with the target DNA. This disclosure provides a method of modulating the transcription of a target nucleic acid in a target cell and generally involves contacting the target nucleic acid enzymatically with an inactive Cas9 polypeptide and DNA-targeted RNA. Kits and compositions for carrying out the method are also provided. This disclosure discloses genetically modified cells producing Cas9; And Cas9 grafted non-human multi-cellular organisms.