A C A A BOTULINUM TOXIN TYPE A VACCINE COMPRISING HEAVY CHAIN C-TERMINAL OF BOTULINUM TOXIN TYPE A MUTANT INHIBITING TOXIC ACTIVITY AND METHOD THEREOF

摘要

The present invention relates to a botulinum toxin type A vaccine which has an eliminated toxic activity site and a suppressed cell binding activity by substituting arginine (Arg) 1269, tyrosine (Tyr) 1117, histidine (His) 1253, tryptophan (Trp) 1266, and glutamate (Glu) 1203 that are present in a cell binding region from threonine (Thr) 876 to leusine (Leu) 1296, which corresponds to the heavy chain C-terminal (Hc) domain of botulinum toxin type A, with alanine (Ala); and to a method of producing the same. According to the present invention, the vaccine may be used as a composition for preventing and treating diseases caused by botulinum toxin type A poisoning.