The present invention relates to a microalgae homogenate preparation step for preparing microalgae homogenate for more efficient and high performance microalgae gene function analysis; Supporting the microalgae homogenate in a reaction vessel and treating an effector molecule; And measuring the biological effect according to the treatment of the effect molecule, as compared to a control group in which the effect molecule is not treated. The present invention also provides a method for assaying metabolic pathway using a microalgae cell-free system.
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