For more efficient and high-performance analysis of a function of a microalgae gene, the present invention provides an empirical analysis method of a metabolic pathway using a microalgae cell-free system, including: preparing microalgae homogenate; allowing a reaction vessel to contain the microalgae homogenate, and then treating with an effector molecule; and comparing with a control, which is not treated with the effector molecule, to measure a biological effect of the effector molecule treatment.
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