Method for manufacturing patient-specific anti-cancer therapeutic agent and method for treating the same

摘要

(a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading a dissociated cell sample into the microfluidic device having at least one isolated region fluidly connected to the flow region and the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolated region in the microfluidic device; And (d) using a microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. Cancer cells can be the patient's own cancer cells. Also provided are methods of treating a patient, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.