- - Method for generating induced pluripotent stem cells from human-derived somatic cells using reprogramming-enhancing agents

摘要

The present invention relates to a method for producing a recombinant human somatic cell, comprising the steps of: (a) culturing a human-derived somatic cell into which a gene encoding a dediffering inducer is introduced in the presence of at least one selected from the group consisting of a protein kinase C inhibitor, a histone deacetylase inhibitor, Culturing in a medium containing a differentiation-enhancing agent; And (b) separating embryonic stem cell-like colonies from the culture obtained from step (a). The present invention also provides a method for producing iPS cells. The production method of the present invention is capable of producing reprogrammed pluripotent stem cells at high efficiency and also capable of producing reprogrammed pluripotent stem cells under xenopathogen-free and feeder cell-free conditions Can be manufactured.