TSPYL5 120 Method to block the growth of cancer stem cells via inhibiting the phosphorylation of 120th threonine residue in TSPYL5 and Peptide inhibitor and the use thereof

摘要

The present invention relates to a peptide which inhibits phosphorylation of the 120th threonine residue of TSPYL5 (Testis-specific Y-like protein 5) protein. The T120D and T120A-TSPYL5 mutations of the 120th threonine (T120) residue of TSPYL5 were transformed into the cells to confirm the effect of phosphorylation of T120 residues. As a result, normal TSPYL5 and T120D migrated into the nucleus However, in the case of T120A, it was confirmed that TSPYL5 protein did not migrate into the nucleus but was expressed only in cytoplasm, could not bind AKT, increased ubiquitination of TSPYL5 protein and suppressed sumoylation, ALDH1-A1, -A3, CD44 gene and protein expression, decreased the growth and metastasis of lung cancer cells, and decreased sphere formation. Based on this, a peptide consisting of the amino acid sequence of SEQ ID NO: 43 or SEQ ID NO: 44 of the present invention which inhibits the phosphorylation of the 120th threonine residue of TSPYL5 has been devised and they have been useful as cancer stem cell growth or metastasis or cancer stem cell growth inhibitor composition Can be used.