A method for simultaneously detecting and identifying Gram positive bacteria Gram negative bacteria Candida species and antibiotics-resistance and a kit therefor based QuantaMatrix assay Platform

摘要

The present invention relates to a method capable of rapidly distinguishing gram-positive bacteria, gram-negative bacteria, and Candida species and identifying resistance of each gene corresponding to MRSA and VRE, ESBLs (TEM-, CTX-M-, and SHV-type), AmpC (ACT, CMY2, DHA, CMY-1 like/MOX, ACC-1, and FOX), and carbapenemases (OXA-48 like, IMP, VIM, NDM, KPC, and SPM) and a primer and a probe used therefor. The method comprises the steps of: a) separating DNA from a specimen sample; b) performing PCR amplification from the DNA using the primer of a sequence number 1-56; and c) hybridizing PCR amplification a disk to which oligomer probe of a sequence number 57-117 coupled with a product obtained in the step b) and then measuring an image of the disk through a Quanta Matrix assay Platform software.