NANOTUBE ARRAY WITH FLUORESCENT MATERIAL INCLUDED THEREIN AND PEPTIDE CAP HAVING SUBSTRATE SPECIFICITY TO PROTEASE ON OUTER SURFACE, MANUFACTURING METHOD THEREOF, AND QUANTIFYING METHOD OF PROTEASE BY FLUOROMETRY USING SAME

摘要

The present invention relates to a nanotube array with a fluorescent material included therein and a peptide cap having substrate specificity to a protease on an outer surface, a manufacturing method thereof, and a quantifying method of a protease by fluorometry using the same. The present invention provides a nanotube array in which a peptide cap degradable by an enzyme is formed on a nanotube. According to the present invention, the nanotube array has a densely introduced hydrophobic cap material (peptide cap) with substrate specificity to an enzyme to properly store a fluorescent material therein. Also, the hydrophobic cap material is degraded when coming in contact with the enzyme to discharge the fluorescent material out of the nanotube. If a fluorescence intensity value of a collected dye is measured after coming in contact with the enzyme (incubation) for at least two hours, the trypsin concentration can be detected in a range of 1 ng. Quantification of a protease can be realized without a process of undergoing centrifugation of a reaction solution.;COPYRIGHT KIPO 2018