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A Novel Enterococcus species specific primer a method for isolating and identifying specific Enterococcus strain by using the same and a composition therefor

机译：新型肠球菌种特异性引物，使用其鉴定和鉴定特定肠球菌菌株的方法及其组合物

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摘要

The present invention relates to a Multiplex-PCR method for selecting Enterococcus strains, and more particularly, to a Multiplex-PCR method using Enterococcus 4 species (E. faecalis, E. faecium, E. hirae, E. durans) . The present invention uses a newly designed computer programming analysis technique to search for a gene specifically present in each species of Enterococcus and to use it to prepare a primer and to perform PCR, PCR method, it is possible to identify Enterococcus 4 species ( E.faecalis, E.faecium, E.hirae, E.durans ) by a simple and economical method. In addition , it is possible to analyze and apply other genus phases such as Lactobacillus and Bacillus as well as Enterococcus based on the newly designed programming tool. It is time-saving and economical in development of probiotics and feed additives that devote most time to isolate and identify bacteria. to be.

机译：本发明涉及选择肠球菌菌株的多重PCR方法，更具体地，涉及使用肠球菌4种（粪肠球菌，粪肠球菌，平肠埃希氏菌，杜兰肠球菌）的多重PCR方法。本发明使用一种新设计的计算机程序分析技术来搜索在每种肠球菌中特异性存在的基因，并用它制备引物并进行PCR， PCR方法可鉴定肠球菌4 种类（ E。粪肠球菌，E。粪便，E。 hirae ，E。 durans ）。除了，还可以基于新设计的编程工具分析和应用其他属相，例如乳酸杆菌和芽孢杆菌以及肠球菌。益生菌和饲料添加剂的开发既省时又经济，它们可以将大部分时间用于分离和鉴定细菌。成为。 展开▼

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公开/公告号KR101869832B1

专利类型

公开/公告日2018-06-21

原文格式PDF

申请/专利权人 강원대학교산학협력단;
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申请/专利号KR20160067130

发明设计人 김은배;박종빈;진귀득;
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申请日2016-05-31

分类号G06F19/28;C12Q1/68;G06F19/24;

国家 KR

入库时间 2022-08-21 12:37:40

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1. MALDI‑TOFMS技术鉴定临床感染猪肠球菌的方法 [P] . 中国专利： CN107219293A . 2017-09-29

2. 检测肠球菌耐药性的组合物及方法 [P] . 中国专利： CN103484538B . 2014.12.31

3. A Novel Enterococcus species specific primer a method for isolating and identifying specific Enterococcus strain by using the same and a composition therefor [P] . 韩国专利： KR20170135351A . 2017-12-08

机译：新型肠球菌种特异性引物，使用其鉴定和鉴定特定肠球菌菌株的方法及其组合物

4. Primer set specific for vancomycin-resistant Enterococcus, composition comprising the same and method of detecting vancomycin-resistant microorganism Enterococcus in sample [P] . 美国专利： US9487833B2 . 2016-11-08

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5. PRIMER SET SPECIFIC FOR VANCOMYCIN-RESISTANT ENTEROCOCCUS, COMPOSITION COMPRISING SAME, AND METHOD FOR DETECTING VANCOMYCIN RESISTANT ENTEROCOCCUS IN SAMPLE [P] . 韩国专利： KR20150104892A . 2015-09-16

机译：耐万古霉素肠球菌特异的底漆组合物，其组成相同，以及用于测定样品中耐万古霉素肠球菌的方法

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3. Species-specific COI primers for rapid identification of a globally significant invasive pest, the cassava mealybug Phenacoccus manihoti Matile-Ferrero [J] . WANG Yu-sheng, TIAN Hu, WAN Fang-hao, 农业科学学报（英文版） . 2019,第005期

4. Species-specific COI primers for rapid identification of a globally significant invasive pest, the cassava mealybug Phenacoccus manihoti Matile-Ferrero [J] . WANG Yu-sheng, TIAN Hu, WAN Fang-hao, 农业科学学报：英文版 . 2019,第005期

5. Development of Species-Specific PCR Primers and Sensitive Detection of the Tylenchulus semipenetrans in China [J] . LIU Guo-kun, CHEN Juan, XIAO Shun, 农业科学学报（英文版） . 2011,第002期

6. Rapid identification of Enterococcus faecalis by species-specific primers based on the genes involved in the Entner-Doudoroff pathway [J] . Peykov Slavil Z., Aleksandrova Vanya D., Dimov Svetoslav G. Molecular biology reports . 2012,第6期

7. Clinical isolates of Enterococcus faecium exhibit strain-specific collagen binding mediated by Acm, a new member of the MSCRAMM family [J] . Nallapareddy SR., Weinstock GM., Murray BE. Molecular Microbiology . 2003,第6期

8. CRISPRs of Enterococcus faecalis and E. hirae Isolates from Pig Feces Have Species-Specific Repeats But Share Some Common Spacer Sequences [J] . Katyal Isha, Chaban Bonnie, Ng Beata, Microbial Ecology: An International Journal . 2013,第1期

9. Design of β-hairpin pepticles with potent antimicrobial activity and target specificity against Enterococcus faecalis [C] . Lin Xu, Anshan Shan, Changxuan Shao, World biomaterials congress . 2016

10. Evaluation of water quality parameters (Enterococcus spp., total suspended solids and specific conductance) in harvested rainwater and implications for management and use. [D] . Wooten, Leslie Ann. 2015

11. Designation of the Provisional New Enterococcus Species CDC PNS-E2 as Enterococcus sanguinicola sp. nov. Isolated from Human Blood and Identification of a Strain Previously Named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina Ricci Mora and Manachini 2004 [O] . Maria da Glória S. Carvalho, Arnold G. Steigerwalt, Roger E. Morey, 2008

12. 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Vascular304Mitophagy acts as a safeguard mechanism against human vascular smooth muscle cell apoptosis induced by atherogenic lipidsTranscriptional control and RNA species - Vascular307MicroRNA-34a role in vascular calcification308Local delivery of a miR-146a inhibitor utilizing a clinically applicable approach attenuates neointima formation after vascular injury309Long noncoding RNA landscape of hypoxic endothelial cells310Specific circulating microRNAs levels associate with hypertension, hyperglycemia and dysfunctional HDL in acute coronary syndrome patientsCytokines and cellular inflammation - Vascular313Phosphodiesterase5A up-regulation in vascular endothelium under pro-inflammatory conditions: a newly disclosed anti-inflammatory activity for the omega-3polyunsaturated aatty acid docosahexaenoic acid314Cardiovascular risk modifying with extra-low dose anticytokine drugs in rhematoid arthritis315Conversion of human M-CSF macrophages into foam cells reduces their proinflammatory responses to classical M1-polarizing activation316Lymphocytic myocarditis coincides with increased plaque inflammation and plaque hemorrhage in coronary arteries, facilitating myocardial infarction317Serum osteoprotegerin level predictsdeclined numerous of circulating endothelial- derived and mononuclear-derived progenitor cells in patients with metabolic syndromeGrowth factors and neurohormones - Vascular320Effect of gastrin-releasing peptide (GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325Regulation of beta-adrenoceptor-evoked inotropic responses by inhibitory G protein, adenylyl cyclase isoforms 5 and 6 and phosphodiesterases326Binding to RGS3 and stimulation of M2 muscarinic acetylcholine receptors modulates the substrate specificity of p190RhoGAP in cardiac myocytes327Cardiac regulation of post-translational modifications, parylation and deacetylation in LMNA dilated cardiomyopathy mouse model328Beta-adrenergic regulation of the b56delta/pp2a holoenzyme in cardiac myocytes through b56delta phosphorylation at serine 573Nitric oxide and reactive oxygen species - Vascular331Oxidative stress-induced miR-200c disrupts the regulatory loop among SIRT1, FOXO1 and eNOS332Antioxidant therapy prevents oxidative stress-induced endothelial dysfunction and Enhances Wound Healing333Morphological and biochemical characterization of red blood cell in coronary artery diseaseCytoskeleton and mechanotransduction - 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V Garcia-Martinez, C Lopez Sanchez, W Hamed, 2016

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