The present invention relates to a scaffold assembly for in vitro expansion of hematopoietic stem cells or progenitor cells, a perfusion type bioreactor for in vitro expansion of hematopoietic stem cells or progenitor cells using the scaffold assembly, and a biological reaction system. The scaffold assembly of the present invention and the flow-through reactor using the scaffold assembly of the present invention can be produced by using a material having biocompatibility, chemical compatibility and mechanical compatibility, A scaffold for providing an environmental environment, and a nanosheet, which are structurally similar to osteoblastic niche, as well as cells of a fixed size by the pump without loss of cells fixed to the scaffold The cells fixed to the scaffold are exposed to a certain stimulus to provide an optimal environment similar to that in vivo for in vitro growth of hematopoietic stem cells or progenitor cells.
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