METHOD FOR DETERMINING THE HUMAN GENOTYPE BY POLYMORPHISM IN THE CYTOCHROME P450 GENE CYP2D6*4 (1846GA), RS3892097

摘要

FIELD: medicine; biotechnology.SUBSTANCE: invention relates to medicine, biology and biotechnology and is intended to determine the human genotype by polymorphism in the cytochrome P450 gene CYP2D6*4 (1846GA) rs3892097. Pair of primers is used, fluorescently labeled allele-specific oligonucleotide probes, and a universal oligonucleotide labeled with a fluorescence quencher are used. Assignment of a sample to a homozygote or heterozygote for a given allele is evaluated by the shape of the DNA melting curves, namely by the maximum of the first derivative of fluorescence plots.EFFECT: invention allows to conduct a study in a single tube, which reduces the cost of the study.1 cl, 1 dwg, 2 tbl