METHOD OF OBTAINING CONDITIONED MEDIA WITH REGENERATIVE POTENTIAL FOR INTRANASAL INTRODUCTION IN TREATMENT OF DISEASES OF CENTRAL NERVOUS SYSTEM

摘要

FIELD: medicine.;SUBSTANCE: conditioned media is obtained by laboratory processing and cultivation of type 2 macrophages obtained from the adherent fraction of peripheral blood mononuclear cells of the patient or his immediate relative by cultivation for 7-8 days in a nutrient medium supplemented with 2-3% autologous plasma in the presence of granulocyte-macrophagal colony-stimulating factor, as a result of which the bioactive factors are secreted to conditioned media, selected from the group consisting of erythropoietin [EPO], insulin-like growth factor-1 [IGF-1], interleukin-6 [IL-6], brain-derived neurotrophic factor [BDNF], epidermal growth factor [EGF], basic fibroblast growth factor [FGF-basic]. Conditioned media is used in the form of intranasal inhalations according to the individual course program, depending on the patient's initial condition and the features / severity of the disease.;EFFECT: conditioned medium obtained by the proposed method has a regenerative potential in the treatment of functional disorders of central nervous system and the consequences of organic brain damages.;3 cl, 4 ex