RECOMBINANT PLASMID pAL2-T-WBMAG DNA, USED FOR CREATION OF DNA-CALIBRATORS AT THERAPY EFFICIENCY ASSESSMENT FOR PATIENTS WITH ACUTE MYELOID LEUKEMIA

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to recombinant plasmid DNA. The recombinant plasmid pAL2-T-WBMAG DNA is developed, including fragments of WT1, BAALC, MN1, ABL1 and GUSB genes. The invention is used as calibrators in RT-PCR and real-time detection of genespecific products in order to compare the expression of these genes in patients with acute myeloid leukemia (AML). WT1, BAALC and MN1 genes act as tumour progression markers, and ABL1 and GUSB genes - as expression normalizers.;EFFECT: invention allows to increase the assessment accuracy of the relative expression of these genes due to the DNA calibrators design that combine sequences of all markers, complex analysis of the WT1, BAALC and MN1 genes allows to evaluate therapy effectiveness in most cases of acute myeloid leukemia, including in patients without detected chromosomal aberrations.;4 dwg