METHOD FOR PRODUCTION OF VACCINE AGAINST BRUCELLOSIS OF SMALL CATTLE

摘要

FIELD: biotechnology.;SUBSTANCE: method for production of vaccine against the brucellosis of small cattle provides seeding and growing strain of Brucella melitensis Rev-1 on a nutritional medium, containing pancreatic digest of casein of a high decomposition degree, yeast extract, sodium chloride, glucose, glycerol, and agar-agar in the specified component ratio within 72 hours at 37°C. The grown vaccine culture is washed from the surface of the agar by a drying medium and brought to a concentration of 80-140 billion m.c. per 1 ml. It is checked for purity and dissociation, packed in ampoules and subjected to freeze-drying.;EFFECT: increase in the yield of Brucella melitensis Rev-1 strain in the S-form.;1 tbl, 1 ex