METHODS AND KITS FOR DETECTION OF CELL-FREE NUCLEIC ACIDS SPECIFIC FOR PATHOGENES

摘要

FIELD: biotechnology.;SUBSTANCE: method for detection of a target nucleic acid derived from Mycobacterium tuberculosis (TB) H37Rv DNA sequence, selected from the group consisting of IS6110, IS1084, MPT 64, rrs, esat6 esat6-like, MDR, rpoB, katG, iniB and their fragments, from the subject. The method includes amplification of the nucleic acid sequence of the target nucleic acid by a pair of primers, where the target nucleic acid is produced from a cell-free blood sample fraction, pleural exudate and/or cerebrospinal liquids (CSL) of the subject.;EFFECT: method results in obtaining of a double-chain DNA, and detection of the double-chain DNA, where the presence of the double-chain DNA indicates the presence of the target nucleic acid in the subject.;16 cl, 5 dwg, 2 tbl, 4 ex