MUTANT RECOMBINANT HEPARINASE I WITH INCREASED SPECIFIC ACTIVITY FROM PEDOBACTER HEPARINUS, DNA FRAGMENT THAT CODES THE SAID HEPARINASE

摘要

FIELD: biotechnology.;SUBSTANCE: group of inventions relates to biotechnology. Variant of the mutant recombinant heparinase I from Pedobacter heparinus containing the following amino acid substitutions as compared to the amino acid sequence of the initial heparinase I: E117Q, Q118P, E362P, Y363Pis proposed. DNA fragment encoding the said mutant heparinase I is proposed.;EFFECT: group of inventions makes it possible to obtain recombinant mutant heparinase I, which has an increased specific activity as compared to the initial unmodified heparinase I.;2 cl, 4 ex