Solanum dubium - seeds and honey (extracted from bees sticks from a acacias - tree - bevölkertem field) combination for the treatment of asthma and bronchial asthma

摘要

As a vegetable drug for asthma, the invention is composed of a weighed out proportional mixture of sunnut honey and powders of milled seeds of solanum dubium.A phytochemical analysis was carried out on the crude extracts of the seeds, honey and their combination is carried out. Collection and separation of active substances from the methanolic extract of the combination was with the use of a bioassay - guided fractionation is carried out. Fractionation and separation of the active substances of the methanolic extract were monitored by means of column chromatography (carried out by means of dc and plc). Nuclear magnetic resonance (nmr), mass spectrometry (ms), ultraviolet (uv) - and infrared (ir) - spectrometry were used for the structural elucidation of the insulated compounds are used.The implemented in - vivo experiments are, for this purpose, since, in order to allow the toxicological untersuchug of the raw - and methanolic extracts of the combination, honey and of the seeds on (96) to assess wister albino with the use of the acute toxicity (ld₅₀ Percentage), carried out with 7 - days and subchronic for 28 days. The results showed that, in the case of the different concentrations of the extracts no acute or subchronic toxicity is placed on the day. The method of a carrageenan - induced - paws edema on (162) wister - rats was carried out in order the anti-inflammatory effects of an aqueous crude extract by s. dubuim - seeds, honey and the combination to judge. The results showed that all the ingredients, a significant anti-inflammatory activity on the animal's paws edema circumference have.Likewise, the results for methanolic extracts a significant inflammation inhibiting. The results of the anti-inflammatory activity of the fractions of a methanolic extract, five fractions showed a significant inflammation inhibiting with respect to the other extracts alcoholic fractions. It is also reduced all fractions rats paws edema.The bronchodilating agent is - activity of an aqueous crude extract of the three components was carried out with the use of in vitro studies on isolated tracheal preparations of guinea pigs and guinea pigs - pattern of a test set-up for allergic asthma. The results of bronchodilator - activity, the animal of the control group, before administration of the 1mg / ml of combination blocked partially the contracting effect of 100 nano g / ml of histamine on isolated in a spiral cut air tubes strips. The page dubuim - seeds - extract produced by a further transient stimulating effect followed by a continuous relaxing effect on the histamine - contracting effect on isolated guinea pigs - air tubes strips. In animals of the sensitized group consisting of the relaxed the combination and 1mg / ml of seeds - extract effectively prevents the stimulating effect of the guinea pigs - air tubes strip. The relaxing effect of the combination was completely blocked by propranolol. The sensitized and the treatment - sub-groups, honey, as well as the combination of damage both the contracting effect of 100 nano g / ml of histamine on guinea pigs - air tubes strips against.As a clinical study for the combined ingredients were twenty-five patient, the medically as asthmatics are diagnosed, arbitrarily selected and for the study; none of them an antiasthmatic medicaments currently used. Characters of asthma disappeared in the case of the treated patient.