Method for generating a three-dimensional model of a sample in a digital microscope and digital microscope

摘要

The present invention relates to a method for generating a three-dimensional model of a sample (09) using a microscope (01), comprising the following steps: specifying a perspective for recording images of at least one area of the sample (09), wherein the perspective is specified by the angle and the position of the optical axis of the objective lens relative to a sample, and by the angular distribution of illumination radiation relative to the sample; recording multiple individual images of the sample (09) at various focus positions from the specified perspective; repeating the preceding steps for the at least one area of the sample (09) with at least one other different perspective; computing a three-dimensional model of the area of the sample (09) from the recorded individual images of the area of the sample (09). The invention further relates to a digital microscope that is configured for carrying out the method according to the invention.