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METHOD FOR EVALUATING BOTH INDUCTION OF DRUG-METABOLIZING ENZYME AND CYTOTOXICITY, AND VECTOR AND CELL FOR USE IN SAID METHOD
METHOD FOR EVALUATING BOTH INDUCTION OF DRUG-METABOLIZING ENZYME AND CYTOTOXICITY, AND VECTOR AND CELL FOR USE IN SAID METHOD
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机译:评价药物代谢酶和细胞毒性两者的诱导方法以及用于上述方法的载体和细胞
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摘要
It is an object of the present invention to evaluate the induction of a drug-metabolizing enzyme by a test substance more accurately than ever before, by simple operations, using a vector in which a reporter gene is connected downstream of the expression control region of a drug-metabolizing enzyme gene and a cell into which the aforementioned vector is introduced. The present invention provides a vector for producing a cell that is used for evaluation of the induction of a drug-metabolizing enzyme by a test substance and the cytotoxicity of the test substance, the vector comprising an expression control region of a fetus-specific gene, a first reporter gene, an expression control region of a drug-metabolizing enzyme gene, and a second reporter gene, wherein the first reporter gene is located downstream of the expression control region of the fetus-specific gene and the second reporter gene is located downstream of the expression control region of the drug-metabolizing enzyme gene. In addition, the present invention also provides a cell comprising the aforementioned vector and a method for evaluating induction of a drug-metabolizing enzyme by a test substance and cytotoxicity of the test substance using the vector.
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