METHOD FOR SUPPRESSING NONSPECIFIC SIGNALS FROM CONTAMINANTS IN AN IMMUNOASSAY USING SURFACE PLASMON-FIELD ENHANCED FLUORESCENCE SPECTROSCOPY (SPFS)

摘要

An object of the present invention is to provide a method of suppressing, in an immunoassay using surface plasmon-field enhanced fluorescence spectroscopy (SPFS), nonspecific signals generated by nonspecific adsorption of contaminants contained in a sample to an SPFS sensor section (e.g., a primary antibody, a solid-phase layer and a metal thin film). The present invention relates to a method of suppressing nonspecific signals originating from contaminants in an immunoassay using surface plasmon-field enhanced fluorescence spectroscopy (SPFS) (including cases where a receptor for a compound to be measured is used in place of a primary antibody), the method comprising performing at least one of the following pretreatments: (1) a pretreatment of adding an acid or an alkali to a sample; (2) a pretreatment of adding a metal ion to the sample; and (3) a pretreatment of heating the sample.