The present invention relates to a method a method for simultaneous determination of microparticle (or micro particle, respectively) concentration and their morphological and physiological traits in a sample, the method comprising the steps of providing said sample in a sensing chamber with a detection system, exposing said sample to predefined excitation wavelengths in said sensing chamber, simultaneously detecting of emitted signals of said sample in reaction of said exposing step, calculating an average signal intensity on the basis of the detected signals; and determining the microparticle concentration of said sample and their morphological and physiological traits in a simultaneous manner on the basis of said average signal intensity.
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