MUTANT MICROORGANISMS TO SYNTHESIZE COLANIC ACID, MANNOSYLATED AND/OR FUCOSYLATED OLIGOSACCHARIDES

摘要

The present invention relates to mutated and/or transformed microorganisms for the synthesis of various compounds. More specifically, the present invention discloses microorganisms mutated in the genes encoding for the regulators ArcA and IcIR. The latter mutations result in a significant upregulation of the genes that are part of the colanic acid operon. Hence, said microorganisms are useful for the synthesis of any compound being part of the colanic acid pathway such as GDP-fucose, GDP-mannose and colanic acid, and/or, can be further used - starting form GDP-fucose as a precursor- to synthesize fucosylated oligosaccharides or - starting from GDP-mannose as a precursor - to synthesize mannosylated oligosaccharides. In addition, mutations in the genes coding for the transcriptional regulators ArcA and IcIR lead to an acid resistance phenotype in the exponential growth phase allowing the synthesis of pH sensitive molecules or organic acids.