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PROGRAMMABLE RNA SHREDDING BY THE TYPE III-A CRISPR-CAS SYSTEM OF STREPTOCOCCUS THERMOPHILUS

机译：嗜热链球菌的III-A型CRISPR-CAS系统进行的可编程RNA切碎

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摘要

A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.

机译：包含crRNA，Csm4和Csm3的嗜热链球菌的III-A型CRISPR-Cas（StCsm）复合物，用于体外或体内裂解带有与crRNA互补的核苷酸序列的RNA。公开了使用包含crRNA，Csm4和Csm3的最小复合物的RNA引导的RNA内切核酸酶进行靶RNA分子的位点特异性切割/切碎的方法，以及RNA敲除和RNA敲除的方法。

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公开/公告号EP3189140B1

专利类型
公开/公告日2019-10-23

原文格式PDF
申请/专利权人 VILNIUS UNIVERSITY;
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申请/专利号EP20150774716
发明设计人 SIKSNYS VIRGINIJUS;KAZLAUSKIENE MIGLE;TAMULAITIS GINTAUTAS;
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申请日2015-09-04
分类号C12N15/11;C12N15/113;C07K14/315;C12N9/22;
国家 EP
入库时间 2022-08-21 12:30:33

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