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METHOD FOR SEPARATING AND CULTURING MESENCHYMAL STEM CELLS FROM WHARTON'S JELLY TISSUE OF UMBILICAL CORD
METHOD FOR SEPARATING AND CULTURING MESENCHYMAL STEM CELLS FROM WHARTON'S JELLY TISSUE OF UMBILICAL CORD
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机译:沃顿脐带胶质组织分离和培养间充质干细胞的方法
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摘要
Provided is a method for separating and extracting mesenchymal stem cells from the human umbilical cord. The method uses healthy neonatal umbilical cord tissue; after cleaning and disinfection, mechanically pulverising same, separating the Wharton's jelly, and after treating with erythrocyte lysate, carrying out suspension culture in a serum-free culture medium. Replacing the liquid every 3-5 days; after the plate adherence rate reaches 30-70%, carrying out trypsin digestion, and then collecting the cells by centrifugation for passage amplification, until the rate of confluence of the cells reaches 80-90% confluence, thereby obtaining high purity umbilical cord mesenchymal stem cells.
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