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AN INTEGRATED PCB-BASED MICRODEVICE FOR SENSITIVE NUCLEIC ACID DETECTION, AND METHOD FOR ITS PRODUCTION
AN INTEGRATED PCB-BASED MICRODEVICE FOR SENSITIVE NUCLEIC ACID DETECTION, AND METHOD FOR ITS PRODUCTION
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机译:一种用于敏感核酸检测的基于PCB的集成微设备及其生产方法
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摘要
An integrated microdevice (11) for amplification and detection of nucleic acids comprises a microreactor for nucleic acid amplification in the form of a microfluidic amplification microchamber (1) or in the form of a microchannel, fabricated in one layer of a multilayer PCB structure, a detection microchamber (2) for electrochemical biosensing with a sensing noble-metal plated electrode array (3) allowing the effective immobilization of nucleic acid target probes and electronic quantification of the previously amplified target sequences, fabricated in the same PCB layer as the said microreactor, a microchannel network for delivering the amplified nucleic acid to the said sensing electrode array (3), fabricated in the same PCB layer as the said microreactor, and resistive microheaters (4) for providing the heating zones for nucleic acid amplification and/or denaturation, fabricated in a PCB layer under the said microreactor and/or the said sensing electrode array (3).
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