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ASSAY FOR DETECTING TOTAL AND S129 PHOSPHORYLATED ALPHA-SYNUCLEIN
ASSAY FOR DETECTING TOTAL AND S129 PHOSPHORYLATED ALPHA-SYNUCLEIN
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机译:测定总和S129磷酸化突触核蛋白的方法
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摘要
The invention provides methods of detecting both total alpha-synuclein and S129 phosphorylated (pS129) alpha-synuclein using a donor complex, a first acceptor complex, and a second acceptor complex. The donor complex comprises an antibody that specifically binds to alpha-synuclein linked to a support and a donor label. The 4B12 antibody is an example of a suitable antibody in the donor complex. The first acceptor complex for detecting pS129 alpha-synuclein comprises an antibody that preferentially binds to pS129 alpha-synuclein linked to a support and a first acceptor label. The 11A5 antibody is an example of a suitable antibody in the first acceptor complex. The second acceptor complex for detecting alpha-synuclein comprises an antibody that binds to alpha-synuclein regardless of S129 phosphorylation. The LB509 antibody is an example of a suitable antibody in the second acceptor complex. Because both total alpha-synuclein and pS129 alpha-synuclein can be detected simultaneously, qualitatively or quantitatively, it is a useful tool to study human alpha-synuclein phosphorylation events in biospecimens.
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