首页>
外国专利>
(S)-CARBONYL REDUCTASE HETEROPOLYMERS AND APPLICATION THEREOF IN CATALYSIS OF POLYBENZENE RING COMPOUNDS
(S)-CARBONYL REDUCTASE HETEROPOLYMERS AND APPLICATION THEREOF IN CATALYSIS OF POLYBENZENE RING COMPOUNDS
展开▼
机译:(S)-羰基还原酶杂聚物及其在聚苯环化合物催化中的应用
展开▼
页面导航
摘要
著录项
相似文献
摘要
Provided are novel hetero-oligomers of (S)-carbonyl reductases and their application in catalyzing the reduction of polyphenyl ketones. Also provided are recombinant strains expressing hetero-oligomers of SCR/SCR2 or SCR2/SCR3, which can catalyze the reduction of polyphenyl ketones. The hetero-oligomer of SCR/SCR2 is capable of catalyzing 2,4-dichlorobenzophenone, 2-naphthalenone and [(E)-2-[3-[3-[2-(7-chloro-2-quinolinyl)ethenyl]phenyl]-3-oxopropyl]benzo-ic acid methyl ester (Keto Easter M) with a specific activity of 4.55 U/mg , 2.43 U/mg and 0.86 U/mg, respectively. The hetero-oligomer of SCR2/SCR3 is capable of catalyzing 2,4-dichlorobenzophenone and [(E)-2-[3-[3-[2-(7-chloro-2-quinolinyl)ethenyl]phenyl]-3-oxopropyl]benzo-ic acid methyl ester (Keto Easter M) with a specific activity of 4.42 U/mg and 1.21 U/mg, respectively. No catalyzing activities for reducing polyphenyl ketones were detected in the naturally existing homo-oligomers of SCR, SCR2 or SCR3, which can catalyze the reduction of monobenzoic cyclic compounds. The invention expands substrate spectrum of naturally existing (S)-carbonyl reductases (SCR, SCR2 and SCR3) and provides a novel type of oxidoreductases for catalyzing the reduction of polyphenyl ketones.
展开▼
机译:提供了(I)S-羰基还原酶的新型杂-低聚物及其在催化聚苯基酮还原中的应用。还提供了表达SCR / SCR2或SCR2 / SCR3的杂聚体的重组菌株,其可以催化聚苯基酮的还原。 SCR / SCR2的杂聚物可以催化2,4-二氯二苯甲酮,2-萘酮和[( E I>)-2- [3- [3- [2-(7-氯- 2-喹啉基)乙烯基]苯基] -3-氧丙基]苯甲酸甲酯(Keto Easter M),比活性分别为4.55U / mg,2.43U / mg和0.86U / mg。 SCR2 / SCR3的杂聚物可催化2,4-二氯二苯甲酮和[( E I>)-2- [3- [3- [2-(7-氯-2-喹啉基)]乙烯基]苯基] -3-氧代丙基]苯甲酸甲酯(Keto Easter M),比活性分别为4.42 U / mg和1.21 U / mg。在天然存在的SCR,SCR2或SCR3均聚物中未检测到还原聚苯酮的催化活性,可催化一苯并环化合物的还原。本发明扩展了天然存在的( S I>)-羰基还原酶(SCR,SCR2和SCR3)的底物谱,并提供了一种新型的氧化还原酶,用于催化聚苯基酮的还原。
展开▼