SINGLE-CHANNEL CHEMILUMINESCENCE MICROFLUIDIC CHIP AND DETECTION METHOD THEREOF

摘要

The present invention discloses a single-channel chemiluminescent micro-fluidic chip, including a chip body, wherein the chip body includes an optical path scanning window, a whole blood filter cavity, a quantification-reaction cavity and a waste liquid cavity, the quantification-reaction cavity is composed of a quantification-reaction pool on a lower portion and a reaction pool cover plate for sealing an opening of the quantification-reaction pool, the quantification-reaction pool is equally divided into three cavities by two partition plates a disposed perpendicular to a fluid flow direction, a labeled antibody is placed in the middle cavity, coated antibodies are placed in the partitioned cavities on two sides, a surface, facing the quantification-reaction pool, of the reaction pool cover plate is equally divided into two cover plate parts by a partition plate b disposed perpendicular to the fluid flow direction, and the coated antibodies are embedded in the cover plate parts. As a result, the reagent operation process is simplified, and meanwhile, the coated antibodies and the labeled antibodies are physically separated to effectively avoid nonspecific binding. Furthermore, the reaction tank is designed into a special structure, so that when fluid flows through the reaction tank, wavy fluid flow is generated to fully mix the coated antibodies and the labeled antibodies, and thus, testing efficiency and sensitivity are effectively improved.