A flask assembly that includes: an Erlenmeyer flask; and a liner bag comprising a single opening the bag sized to fit within the flask and having a thickness from about 0.0254 mm to about 0.508 mm. The liner bag is configured for cell culturing and ease of insertion of the bag into the flask. In addition, the liner bag comprises a gas-permeable, polymeric material. Further, the liner bag can include a pocket adapted to receive a removable flask insertion element and/or at least one foldable seam configured to facilitate insertion of the bag into the flask. The liner bag can also include a vent having a gas permeability that is greater than the gas permeability of the gas-permeable, polymeric material employed in the balance of the bag.
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