MANUFACTURING METHOD OF HUMAN FAT TISSUE-DERIVED SOMATIC STEM CELLS

摘要

To obtain a large amount of human fat tissue-derived somatic stem cells holding high differentiation potency to an extent where the cells can be practically used for regenerative medicine.SOLUTION: There is provided a manufacturing method of human fat tissue-derived somatic stem cells in which human fat tissue-derived cells attached to a solid phase are separated into cells which are easily detached from the solid phase and cells which are difficult to be detached from the solid phase, the separated easily detachable cells are cultured in low oxygen under 7% of oxygen concentration, by which a large amount of human fat tissue-derived somatic stem cells holding high differentiation potency can be obtained to an extent where the cells can be practically used for regenerative medicine.