Method of manufacturing induced pluripotent stem cell originated from human somatic cell

摘要

The present invention is prepared from human embryonic stem cells, gene Oct4, Sox2 gene, Nanog gene and a fourth gene Each step of producing the virus was introduced, the mixture was concentrated and the respective virus to prepare a mixed solution, comprising: preparing a concentrated virus-virus solution was mixed with the virus concentration of the first mixture liquid culture medium, pre-incubated in the first culture container After the suspension of human somatic cell, the floating of the somatic cells and the virus solution was mixed and reaction somatic - preparing a virus mixed solution, wherein the somatic cell-virus mixture was added to a second culture vessel and a second medium, and left by introducing said gene in the somatic cells, and provides a process for the preparation of stem cells comprising culturing the somatic cells the genes introduced.