FLORESCENT CALCIUM SENSOR PROTEINS

摘要

PROBLEM TO BE SOLVED: To provide fluorescent calcium sensor protein with high luminance.;SOLUTION: Disclosed herein is a fluorescent calcium sensor protein comprising an amino acid sequence where each of Asn117 and Leu338 of a specific sequence is substituted with a different amino acid. A fluorescent calcium sensor protein G-CaMP7.09 with significantly high luminance compared to G-CaMP7 is produced by returning a few specific amino acid mutations in RESET tag of G-CaMP7 to original amino acid residue as in G-CaMP2, where G-CaMP7 was obtained by introducing several amino acid mutations into G-CaMP2.;SELECTED DRAWING: None;COPYRIGHT: (C)2019,JPO&INPIT