SHORTENED PURIFICATION PROCESS FOR PRODUCTION OF CAPSULAR POLYSACCHARIDES OF STREPTOCOCCUS PNEUMONIAE

摘要

To provide a shortened process for producing a solution containing substantially purified capsular polysaccharides from a cell lysate broth of Streptococcus pneumoniae.SOLUTION: Ultrafiltration and diafiltration of a clarified S. pneumoniae lysate followed by pH adjustment to less than 4.5, preferably about 3.5, precipitate at least 98% of the proteins in the solution without seriously affecting polysaccharide yield. Furthermore, following the ultrafiltration and diafiltration and acidification to a pH of less than 4.5, filtration using activated carbon precipitates at least 90% of remaining proteins without seriously affecting polysaccharide yield. Exemplary, but non-limiting, S. pneumoniae serotypes that can be purified using the shortened process of the invention are 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F.SELECTED DRAWING: None