Optimization of Fermentation Process for Hyaluronic Acid Production by Streptococcus zooepidemicus

摘要

Hyaluronic acid(HA) is a high molecular weight glycosaminoglycan consisting of alternating D-glucuronic acid and N-acetylglucosamine and plays extremely important roles in many biological processes.In this study,we optimized fermentation process for the production of HA by Streptococcus zooepidemicus ATCC35246,including fermentation broth composition and various fermentation parameters.The experimental results showed that the optimal fermentation broth composition was:glucose 45 g/L,yeast extract 10 g/L,tryptone 12 g/L,KH_2PO_42 g/L,K_2HPO_4·3H_2O 2 g/L,MgSO_4 ·7H_2O2 g/L,and(NH_4)_2SO_40.4g/L.The optimal parameters involved in fermentation was:liquid volume 20%,pH 6.0,rotation speed 180 r/min,fermentation temperature 35 °C,fermentation duration 18 h,CTAB concentration 25 mg/L.Under the optimized conditions,the yield of HA was 0.305 g/L,which was dramatically improved by 43.87%compared to that of 0.212 g/L before optimization.