首页> 外国专利> METHOD FOR STORING DENTAL PULP TISSUE AND METHOD FOR CULTURING STEM CELLS FROM STORED DENTAL PULP TISSUE

METHOD FOR STORING DENTAL PULP TISSUE AND METHOD FOR CULTURING STEM CELLS FROM STORED DENTAL PULP TISSUE

机译:储存牙髓组织的方法和从储存的牙髓组织培养干细胞的方法

摘要

To provide methods for storing dental pulp tissue collected from animal's teeth and to provide simple and effective methods in the isolation culture of stem cells from stored dental pulp tissue.SOLUTION: The present invention provides a method for storing dental pulp tissue comprising chopping pulp tissue collected from a living body so that the maximum length is 2 mm or less; immersing the pulverized dental pulp tissue into a cryoprotective solution so that the weight of the pulp tissue/(the weight of the pulp tissue + the weight of the cryoprotective solution) is 10 wt% or more and less than 50 wt%; and placing the chopped dental pulp tissue and cryoprotective solution in a container and cryopreserving it in an environment of -80°C or less.SELECTED DRAWING: None
机译:提供一种用于存储从动物牙齿收集的牙髓组织的方法,并提供从存储的牙髓组织中分离培养干细胞的简单有效的方法。解决方案:本发明提供一种用于存储牙髓组织的方法,该方法包括将收集的牙髓组织切碎距活体最大长度不超过2毫米;将粉碎的牙髓组织浸入冷冻保护溶液中,以使组织的重量/(组织的重量+保护溶液的重量)为10重量%以上且小于50重量%。将切碎的牙髓组织和冷冻保护溶液放入容器中,并在-80°C或更低的环境中冷冻保存。

著录项

  • 公开/公告号JP2018201340A

    专利类型

  • 公开/公告日2018-12-27

    原文格式PDF

  • 申请/专利权人 TOYOBO CO LTD;

    申请/专利号JP20170106595

  • 发明设计人 YAMAGUCHI TATSUYA;

    申请日2017-05-30

  • 分类号C12N1/04;C12N5/02;C12M1/00;C12M3/00;

  • 国家 JP

  • 入库时间 2022-08-21 12:22:09

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