METHOD FOR PREPARING DENTAL PULP STEM CELLS FROM CELLS DERIVED FROM DENTAL PULP TISSUE

摘要

[Problem] The present invention addresses the problem of providing a method for efficiently preparing dental pulp stem cells from dental pulp cells collected from dental pulp tissue. [Solution Means] The method for preparing dental pulp stem cells from dental pulp cells collected from dental pulp tissue according to the present invention includes: (a) a step for preculturing dental pulp cells using a culture medium that includes serum at 7-13% (v/v), the dental pulp cells being bonded to a culture dish; (b) a step for culturing the dental pulp cells bonded to the culture dish by step (a) at least for one day using a culture medium that includes serum at 7-13% (v/v); and (c) a step for culturing the dental pulp cells in a low-serum condition after step (b).