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ISOLATION METHOD OF DROPLETS FOR GENOMIC ANALYSES AND THE GENOMIC ANALYSIS METHOD

机译:基因组分析的滴液分离方法和基因组分析方法

摘要

PROBLEM TO BE SOLVED: To provide methods for isolating droplets for subjecting to genomic analysis without affecting a subsequent genomic analysis, and to provide genomic analysis methods.SOLUTION: A method of the present invention is a method for isolating droplets for genomic analysis, the method comprising an emulsion formation process, a nucleic acid amplification step, a detection process, and a fractionation step, the emulsion formation step being a step of contacting a sample comprising cells with an emulsion forming solvent to form in the emulsion forming solvent a plurality of droplets comprising cells in the sample, the nucleic acid amplification step being a step of amplifying a target genome comprised in a target cell with respect to the droplets, the detection step being a step of detecting the presence or absence of amplification of the target genome in the droplets, the fractionation step being a step of fractionating droplets in which the amplification of the target genome has been detected, as droplets for genomic analysis comprising an amplification product derived from the target genome of the target cell.SELECTED DRAWING: None
机译:解决的问题:提供分离用于基因组分析的液滴的方法而不影响后续的基因组分析,并提供基因组分析方法。解决方案:本发明的方法是一种分离用于基因组分析的液滴的方法,该方法包括乳液形成过程,核酸扩增步骤,检测过程和分级分离步骤,乳液形成步骤是使包含细胞的样品与乳液形成溶剂接触以在乳液形成溶剂中形成多个液滴的步骤。所述核酸扩增步骤是相对于所述液滴扩增靶细胞中包含的靶基因组的步骤,所述检测步骤是检测所述靶中基因组的扩增的存在或不存在的步骤。液滴分离步骤是分离液滴的步骤,其中目标基因的扩增已检测到ome,为用于基因组分析的液滴,其中包含源自靶细胞靶基因组的扩增产物。

著录项

  • 公开/公告号JP2018183096A

    专利类型

  • 公开/公告日2018-11-22

    原文格式PDF

  • 申请/专利权人 ENPLAS CORP;

    申请/专利号JP20170087235

  • 发明设计人 SUNAGA SHINYA;

    申请日2017-04-26

  • 分类号C12M1;C12N15/09;

  • 国家 JP

  • 入库时间 2022-08-21 12:22:02

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