A novel and efficient method for reprogramming blood cells into induced pluripotent stem cells

摘要

Described herein are methods and compositions related to the generation of induced pluripotent stem cells (iPSCs). An improved technique for establishing highly efficient and reproducible reprogramming using non-integrated episomal plasmid vectors is described. Using the described reprogramming protocol, non-T cells can be consistently reprogrammed with near 100% success rate from non-T cell or non-B cell sources. Further advantages include the use of a defined reprogramming medium E7 and the use of recombinant human L-521, a defined clinically compatible substrate. Generation of iPSCs from these blood cell sources enables iterative generation of the entire genome repertoire, preservation of genome fidelity and enhanced genome stability. [Selection] Figure 3