Endo-S2 mutant as glycosynthase, method for producing glycoprotein, and use of glycoprotein for glycosylation

摘要

The present invention relates to glycoprotein synthesis. The present invention exhibits reduced hydrolytic activity and increased transglycosylation activity for the synthesis of glycoproteins in which the desired sugar chain has been added to a fucosylated or non-fucosylated GlcNAc-IgG acceptor. A recombinant Endo-S2 variant (designated Endo-S2 glycosynthase) is provided. In this way, the present invention allows for the synthesis and reassembly of therapeutic antibodies, thereby increasing the in vivo half-life, lower immunogenicity, enhanced in vivo activity, increased targeting Provides certain biological activities, such as the ability and / or the ability to deliver a therapeutic agent. [Selection] Figure 1