The present invention relates to a method capable of efficiently increasing and culturing a ratio of limbal stem cells in a limbal tissue-derived epithelial cell plate by culturing the limbal tissue on an amniotic slide support. According to the present invention, the ratio of the limbal stem cells in the limbal tissue-derived epithelial cell plate can be increased stably and rapidly, and the limbal tissue-derived epithelial cell plate is transplanted to a limbal stem cell-deficient patient. When you can increase the success rate. [Selection] Figure 1c
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