T cell receptor and B cell receptor repertoire analysis system using one-step reverse transcription template switch PCR

摘要

The present invention relates to a method for analyzing a repertoire of a variable region of a T cell receptor (TCR) or a B cell receptor (BCR) of a subject, comprising: (1) from RNA obtained from the subject, Providing a nucleic acid sample comprising a plurality of types of T cell receptor (TCR) or B cell receptor (BCR) nucleic acid sequences amplified using step reverse transcription template switching PCR; and (2) contained in the nucleic acid sample. And (3) calculating the appearance frequency of each gene or a combination thereof based on the determined nucleic acid sequence, and deriving the TCR or BCR repertoire of the subject. Provide a way.