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Process for separation of prokaryotic DNA from eukaryotic DNA in a whole blood sample
Process for separation of prokaryotic DNA from eukaryotic DNA in a whole blood sample
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机译:全血样品中从原核DNA分离原核DNA的方法
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摘要
A process for separating prokaryotic DNA from eukaryotic DNA in a whole blood sample infected by pathogen is provided. The process includes extracting prokaryotic and eukaryotic DNA from the whole blood sample, contacting the prokaryotic DNA in the whole blood sample with a motif that specifically binds to the prokaryotic DNA, and generating a bound pair. The process also includes separating the bound pair. The whole blood sample is transfected with bacteriophages genetically modified to contain a defined DNA sequence having a selectable marker DNA. The DNA sequence integrates into the prokaryotic DNA, and the motif is an oligonucleotide specific to the defined DNA sequence in the engineered bacteriophages.
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