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Multiplex detection of molecular species in cells by super-resolution imaging and combinatorial labeling
Multiplex detection of molecular species in cells by super-resolution imaging and combinatorial labeling
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机译:通过超分辨率成像和组合标记多重检测细胞中的分子种类
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摘要
Methods and systems are provided for creating molecular barcodes or indicia for cellular constituents within single cells and for resolving such barcodes or indicia with super-resolution technologies such as super-resolution microscopy. By this approach, numerous molecular species that can be measured simultaneously in single cells. It has been demonstrated that multiple mRNA transcripts can be labeled with a spatially ordered sequence of fluorophores, and that barcodes can be resolved. In addition, alternative splicing events can be characterized by identifying and quantifying mRNA isoforms in an individual cell.
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