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Optical analysis device, optical analysis method and computer program for optical analysis using single light-emitting particle detection
Optical analysis device, optical analysis method and computer program for optical analysis using single light-emitting particle detection
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机译:使用单个发光粒子检测的光学分析装置,光学分析方法和计算机程序
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摘要
There is provided an optical analysis technique of detecting light of a light-emitting particle in a sample solution in the scanning molecule counting method using the light measurement with a confocal or multiphoton microscope, for suppressing the scattering in detected results of signals of light of light-emitting particles smaller and achieving the improvement of accuracy. The inventive technique comprises moving the position of a light detection region along a predetermined route for multiple circulation times by changing the optical path of the optical system; detecting light from the light detection region and generating time series light intensity data during the moving of the light detection region and detecting individually a signal indicating light from each light-emitting particle existing in the predetermined route using the time series light intensity data obtained in the circulating movements of the light detection region of multiple times.
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