Method for detecting telomerase via washing-free anchored-extension and telomeric-binding amplification, and kit

摘要

Provided is a new method for telomerase amplification—washing-free anchored-extension and telomeric-binding amplification (WATA). The method comprises: utilizing an anchored telomerase primer to conduct telomere TTAGGGG sequence (“G sequence”) extension; hybridizing with a template probe having a universal PCR primer sequence and six units of telomere CCCTAA sequence (“C sequence”) on the extended G sequence; removing the uncombined template probe via an enzyme digestion reaction; and the template probe combined with the G sequence conducts PCR reaction, the amplification product being a peculiar DNA fragment of a fixed length.